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known as a reliable resource for recombinant
project outsourcing (providing full-service expression & cloning
expertise).
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PX10B
PX10B Competent Cells*
Item
Catalog #
Competency
Format
CHEMICALLY COMPETENT CELLS:
PX10B
961-500
>10
8
CFU/µg DNA
10 x 100µl (1ml)
961-510
>10
9
CFU/µg DNA
20 x 50µl (1ml)
ELECTROPORATION COMPETENT CELLS:
PX10B
961-520
>10
9
CFU/µg DNA
2 x 100µl (0.5ml)
Description:
PX10B competent cells are ideal for generating cDNA libraries using plasmid derived vectors. They contain the lacZ marker
for a-complimentation of the b-galactosidase gene for blue/white color screening. The restriction systems mcrA, mcrBC, mrr,
and hsdRMS are lacking to facilitate the cloning of DNA that contains methylcytosine or 5-hydroxymethylcytosine.
Recombination is minimized by the recA1 and endA1 markers, enhancing the quality and yield of plasmid DNA.
Genotype:
F– mcrA Δ(mrr-hsdRMS-mcrBC)φ80lacZΔM15 ΔlacX74 recA1endA1 araΔ 139 Δ(ara,leu)7697 galU galK λ -rpsL (StrR) nup
G
Storage:
Store at -80 ° C.
Shipping method:
PX10B Competent Cells are packaged with dry ice and shipped overnight.
*Compare to DH10B
